Simiao Qingwen Baidu decoction inhibits Epstein–Barr virus-induced B lymphoproliferative disease and lytic viral replication

Simiao Qingwen Baidu decoction inhibits Epstein–Barr virus-induced B lymphoproliferative disease and lytic viral replication

Blog Article

Context Simiao Qingwen Baidu Kids T-Shirt decoction (SQBD), a traditional Chinese medicine prescription, can ameliorate Epstein–Barr virus (EBV) induced disease.However, its mechanism still remains unknown.Objective To detect the mechanism of SQBD in EBV-induced B lymphoproliferative disease in vitro.Materials and methods Sprague–Dawley (SD) rats (n = 20) were given SQBD (10 mL/kg) by gavage once a day for 7 d.SQBD-containing serum was obtained from abdominal aortic blood of rats, and diluted with medium to obtain 5%, 10% or 20%-medicated serum.

SD rats (n = 10) were given normal saline, and normal serum was collected as a control.EBV-transformed B cells (CGM1) were cultured in medium containing 5%, 10% or 20%-medicated serum.CGM1 cells were treated with normal serum as a control.Cell viability and apoptosis were examined.The expression and activity of proteins were assessed.

Results We found that IC50 (83 ± 26.07%, 24 h; 69.88 ± 4.69%, 48 h) of 10% medicated serum was higher than that of 5% (25.47 ± 6.

98%, 24 h; 21.62 ± 7.30%, 48 h) and 20%-medicated serum (51 ± 7.25%, 24 h; 56.03 ± 2.

56%, 48 h).Moreover, SQBD promoted apoptosis of CGM1 cells by regulating EBV latency proteins expression.SQBD inhibited EBV-induced lytic viral replication.Conclusions T-Shirt Our data confirmed that SQBD inhibits EBV-induced B lymphoproliferative disease and lytic viral replication.This work provides a theoretical basis for the mechanism of SQBD in EBV-induced B lymphoproliferative disease, and SQBD may be an effectively therapeutic drug for EBV-induced B lymphoproliferative disease.